The PR-CLIMB program will incorporate weekly workshops ina hybrid fashion program due to the pandemic situation. The program has been re-designed to instruct students on the operation of state-of-the-art instrumentation and application of modern techniques that will prepare them for developing experimental design and analytical skills useful for their research projects during the summer. The topics of the workshops address the research areas included in the proposal regarding the engineering of materials and biomolecules for potential applications in water purification, energy storage, sensing, drug design, and drug delivery. Learning such techniques will serve a greater purpose: it will empower the REU students in learning about tools and techniques used in the biotech and pharmaceutical industry while preparing them for graduate studies in the fields of biotechnology and materials science. Faculty members of the chemistry graduate program will organize the hands-on workshops offered to PR-CLIMB REU students. Each instructor is an expert in the technique delivered. Our MALDI TOF/TOF workshop was the first one to be implemented as an online workshop:
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List of topics and description
Workshop 1. Sample preparation fundamentals for chromatographic analysis. (Instructor: Dr. Liz Díaz) In this workshop participants will learn about the complex nature of biological sample analysis due to the presence of multiple interferences. Emphasis will be placed on the quality of sample preparation for analysis by chromatographic techniques. These will include: 1. QuEChERS sample preparation 2. Solid Phase Micro Extraction (SPME), 3. Solid Phase Extraction (SPE) 4. Headspace sampling, 5. Pressurized Liquid Extraction, 6. Accelerated Solvent Extraction, 7. Soxhlet Extraction 8. Liquid/ liquid Extraction.
Workshop 2. MALDI TOF TOF Mass Spectrometry. (Instructor: Dr. Tinoco) In this workshop participants will learn about different sample preparations and the selection of appropriate matrices to optimally detect different types of molecules. An emphasis will be made on the detection of parent ions in TOF mode and of fragment ions in TOF TOF mode. Participants will be encouraged to bring samples to evaluate.
Workshop 3. Two-dimensional NMR spectroscopy (Instructor: Dr. Marvin Bayro) Participants will be given an introduction to two-dimensional NMR for structure elucidation, specifically COSY and NOESY. Participants will be introduced to the NMR facility, which includes 500 MHz and 700 MHz instruments.
Workshop 4. Fundamentals of X-ray powder diffraction. (Instructor: Dr. Vilmalí López-Mejias of UPR RP Chemistry) This workshop will introduce participants to X-ray powder diffraction and orient them on the useful elemental and structural information that can be obtained. Participants will learn the basics of sample preparation, sample loading, and data collection and analysis while gaining hands-on experience on a state-of-the-art X-ray diffractometer acquired through the NSF MRI Grant No. CHE-1626103.
Workshop 5. Microscopy. (Dr. Rodríguez Martínez) This workshop will include the basic and advanced uses of a fluorescent microscope. Participants will be able to prepare and visualize fixed cells/tissues or live cells in bright field and use fluorescent markers. A Nikon expert will talk about the image analysis software.
Workshop 6. Assessing the colloidal properties of bio/nanomaterials (Instructor: Dr. Eduardo Nicolau) In this workshop participants will use dynamic light scattering and zeta potential devices to determine critical properties of colloidal structures such as hydrodynamic radius and charge. These properties are of utmost importance to a wide variety of fields including catalysis, pharmaceuticals, and drug delivery.
Workshop 7. Molecular Modeling. (Instructor: Dr. Dalvin Méndez) This workshop will focus on the theoretical and practical aspects of computational chemistry in a computer lab. Emphasis will be placed on the study and application of molecular mechanics, molecular orbital theory, semi-empirical methods, ab-initio methods, and density functional theory. The workshop will provide the basic tools to design computational studies in areas such as molecular structure, electrochemistry, and vibrational spectroscopy.
Our Team working on the design of the new online MALDI TOF/TOF workshop
YouTube link: https://youtu.be/iE9OYaDUxVg
YouTube link: https://youtu.be/7lDOEFv4z64
YouTube link: https://youtu.be/bNYdudE_yOg
YouTube link: https://youtu.be/6-6qGl7N0B0
YouTube link: https://youtu.be/a-sGzsbEm1c
YouTube link: https://youtu.be/7lDOEFv4z64
YouTube link: https://youtu.be/bNYdudE_yOg
YouTube link: https://youtu.be/6-6qGl7N0B0
YouTube link: https://youtu.be/a-sGzsbEm1c